Combination of Hypoosmotic Swelling/Eosin Y Test for Sperm Membrane Integrity Evaluation: Correlations with Other Sperm Parameters to Predict ICSI Cycles

Hypoosmotic Sperm Swelling Test: Reevaluation of Its Potential Use

The aim of this study was to assess the predictive value of the hypoosmotic sperm swelling test. Good correlations were observed between the percentage of spermatozoa undergoing swelling and the percentage motility both in normal (r = 0.68; p < 0.001) and infertile patients (r = 0.65; p < 0.001). In contrast, sperm swelling did not significandy correlate either with sperm oocyte fusion (assesse...

the effect of varicocelectomy on sperm parameters, membrane integrity and sperm chromatin condensation

introduction: varicocele is considered as a common cause of male infertility. the aim of this study was to evaluate the effects of varicocelectomy on sperm parameters, sperm membrane integrity and sperm nuclear maturity. materials and methods: in this study along with routine semen analysis, hypo-osmotic swelling test (host), and aniline blue staining for assessment of as 45 and 90 days after i...

Water-induced hypo-osmotic test for the evaluation of canine sperm membrane integrity

The purpose of this study was to investigate the efficiency of the water test to evaluate the functional membrane integrity of ejaculated canine sperm after collecting, cooling, and freezing-thawing. Twenty-five ejaculates were obtained from 12 stud dogs by digital manipulation. Sperm-rich fractions were evaluated, diluted, cooled at 5oC and frozen-thawed. A conventional hypo-osmotic swelling t...

Intracytoplasmic sperm injection (ICSI).

Intracytoplasmic sperm injection (ICSI)

IVF has become a well-established treatment for certain types of infertility, including long-standing infertility due to tubal disease, endometriosis, unexplained infertility or infertility involving a male factor. Soon it became clear that certain couples with severe male-factor infertility could not be helped by conventional IVF. Extremely low sperm counts, impaired motility and/or abnormal m...